Composite

Part:BBa_K3385064:Design

Designed by: Daniel Bavnh�j   Group: iGEM20_DTU-Denmark   (2020-10-16)


T1G3R_SeglB_glaA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5753
    Illegal EcoRI site found at 7181
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5784
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4015
    Illegal PstI site found at 6591
    Illegal PstI site found at 8325
    Illegal PstI site found at 8926
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5753
    Illegal EcoRI site found at 7181
    Illegal NheI site found at 4892
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4015
    Illegal PstI site found at 6591
    Illegal PstI site found at 8325
    Illegal PstI site found at 8926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5753
    Illegal EcoRI site found at 7181
    Illegal BglII site found at 3870
    Illegal BglII site found at 5392
    Illegal BglII site found at 8423
    Illegal BamHI site found at 1729
    Illegal BamHI site found at 2157
    Illegal BamHI site found at 4850
    Illegal BamHI site found at 5652
    Illegal BamHI site found at 5778
    Illegal BamHI site found at 6329
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
    Illegal XhoI site found at 6189
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5753
    Illegal EcoRI site found at 7181
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5784
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4015
    Illegal PstI site found at 6591
    Illegal PstI site found at 8325
    Illegal PstI site found at 8926
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5753
    Illegal EcoRI site found at 7181
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5784
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4015
    Illegal PstI site found at 6591
    Illegal PstI site found at 8325
    Illegal PstI site found at 8926
    Illegal NgoMIV site found at 2566
    Illegal NgoMIV site found at 5110
    Illegal NgoMIV site found at 8313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3398
    Illegal BsaI site found at 3536
    Illegal BsaI site found at 4225
    Illegal BsaI.rc site found at 4718
    Illegal SapI site found at 8814
    Illegal SapI site found at 9123


Design Notes

Full construct made using non-overlapping primers, which can pose difficulties on PCR amplification and subsequently USER assembly.

Source

Sequences were obtained using aspgd.org[1] and SingalP-5.0[2].

References

[1] Cerqueira GC, Arnaud MB, Inglis DO, Skrzypek MS, Binkley G, Simison M, Miyasato SR, Binkley J, Orvis J, Shah P, Wymore F, Sherlock G, Wortman JR (2014). The Aspergillus Genome Database: multispecies curation and incorporation of RNA-Seq data to improve structural gene annotations. Nucleic Acids Res 42 (1); D705-10.

[2] SignalP 5.0 improves signal peptide predictions using deep neural networks. José Juan Almagro Armenteros, Konstantinos D. Tsirigos, Casper Kaae Sønderby, Thomas Nordahl Petersen, Ole Winther, Søren Brunak, Gunnar von Heijne and Henrik Nielsen. Nature Biotechnology, 37, 420-423, doi:10.1038/s41587-019-0036-z (2019)